Segregation, Mutation, and Copulation in Saccharomyces cerevisiae

TFIIB mutation in Saccharomyces cerevisiae

Mutations in the Saccharomyces cerevisiae SSU71 gene were isolated as suppressors of a transcription factor TFIIB defect that confers both a cold-sensitive growth defect and a downstream shift in transcription start-site selection at the cycl locus. The ssu71-1 suppressor not only suppresses the conditional phenotype but also restores the normal pattern of transcription initiation at cycl. In a...

NDCIO: A Gene Involved in Chromosome Segregation in Saccharomyces cerevisiae

A mutant, ndclO-1, was isolated by antitubulin staining of temperature-sensitive mutant banks of budding yeast, ndclO-1 has a defect in chromosome segregation since chromosomes remain at one pole of the anaphase spindle. This produces one polyploid cell and one aploid cell, each containing a spindle pole body (SPB). NDCIO was cloned and sequenced and is identical to CBF2 (Jiang, W., J. Lechner,...

The Role of H2A.Z in Chromosome Segregation in Saccharomyces cerevisiae

NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae

A mutant, ndc10-1, was isolated by anti-tubulin staining of temperature-sensitive mutant banks of budding yeast. ndc10-1 has a defect chromosome segregation since chromosomes remains at one pole of the anaphase spindle. This produces one polyploid cell and one aploid cell, each containing a spindle pole body (SPD. NDC10 was cloned and sequenced and is identical to CBF2 (Jiang, W., J. Lechnermn ...

Meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae.

Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tetrads with four viable spores, two of the spores had two copies of a given homolog and two spores ...